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Two Distinct Activation Methods Yield Clinical-Scale Expansion of Peripheral Blood Derived γδ T Cell

Download the poster, in which we demonstrate that billions of γδ T cells can be generated from peripheral blood mononuclear cells (PBMCs) with two distinct activation methods, using our TheraPEAK® T-VIVO® Medium – a chemically defined, non-animal origin, serum-free medium, in a rocking motion platform.

Two Distinct Activation Methods Yield Clinical-Scale Expansion of Peripheral Blood Derived γδ T Cells


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Gamma delta (γδ) T cells have inherent ability to infiltrate solid tumors and can directly recognize and kill transformed cells independently of HLA-antigen presentation.

In this study, we first demonstrate robust expansion of billions of γδ T cells (Vδ2+) from cryopreserved PBMCs with zoledronic acid using the TheraPEAK® T-VIVO® Medium supplemented with low amount (100 IU/mL) of IL-2, without the use of human serum.

Moreover, we showcase how the TheraPEAK® T-VIVO® Medium supports the expansion of γδ T cells (including Vδ1+ and Vδ2+ T-cell subsets) by anti-CD3/anti-CD28 co-stimulation, following simple isolation process that depletes αβ T cells and other cells from PBMCs.
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